
3rd International Conference and Exhibition on Metabolomics & Systems Biology
March 24-26, 2014, San Antonio, USA
OMICS International: http://omicsonline.org
Vaccine Conferences: http://conferenceseries.com/immunolog...
Global Medical Conferences: http://conferenceseries.com
Global Pharmaceutical Conferences: http://pharmaceuticalconferences.com
Global Cancer Conferences: http://cancersummit.org
Global Diabetes Conferences: http://diabetesexpo.com
Global Dental Conferences: http://dentalcongress.com
Global Nursing Conferences: http://nursingconference.com
Abstract
Our lab uses an interdisciplinary approach that relies heavily on liquid chromatography–mass spectrometry (LC–MS) based metabolomics to understand medicinally and environmentally relevant microbial processes. The primary analytical platform is an UPLC-Orbitrap MS with electrospray ionization source, and the methods employed attempt to measure the concentration (pool size) of ~1000s of analytically tractable, yet chemically diverse, water and lipid soluble metabolites from all kingdoms of life. These methods measure at least one metabolite from all known carbon and nitrogen utilization pathways, the activated methyl cycle, all amino acid and nucleotide biosynthesis pathways, as well as lipids with diverse head groups; and the average coverage for each major pathway is ~65%. The utility of these metabolomics methods can be enhanced by monitoring the incorporation of stable isotope-labeled nutrient sources, either 15N or 13C, into the metabolome using kinetic flux profiling techniques (KFP). When coupled, pool size determination and KFP can be used to determine both the amounts of metabolites within and relative rates of flux through many biochemical pathways in vivo and allow a global snapshot of cellular metabolism to be obtained from a single set of experiments. Several vignettes from our work studying bacterial cell-cell signaling (quorum sensing), microbial nutrient cycling by marine organisms, and novel biochemical pathways in yeast will be discussed to highlight the utility of applying these metabolomics tools to probe complex biological systems and to provide insight into the mechanisms that consortia of microorganisms utilize to cooperatively and/or competitively dictate resource utilization.
Biography
Shawn R Campagna received his PhD from Princeton in 2006, after working with Prof. Martin Semmelhack on a joint project with Profs. Bonnie Bassler and Frederick Hughson to characterize the chemical properties of an interspecies bacterial signaling molecule, autoinducer-2. He then performed post-doctoral research with Prof. Joshua Rabinowitz at the Lewis-Sigler Institute for Integrative Genomics at Princeton University where he developed mass spectrometric methods for the identification of novel biochemical pathways and natural products from whole cell extracts. He joined the Chemistry Department at UT Knoxville in 2007 where he has worked to develop new metabolomic and lipidomic techniques.
video phone beyonce mp3 Shawn R Campagna| University of Tennessee | USA | Metabolomics 2014 | OMICS International | |
2 Likes | 2 Dislikes |
206 views views | 79 followers |
People & Blogs | Upload TimePublished on 21 Aug 2015 |
Related keywords
profiling for success,video phone commax,free robux,video phone letra,upload to imgur,free ringtones,mass spectrometry services uk,freedom mortgage,video phone number,sharing amazon prime,sharingan eyes,sharing shelf,video phone é a continuação de telephone,upload speed,sharing community,profiling code,mass spectrometry jobs,profiling system,profiling python,profiling adalah,free dating sites,sharing is caring,free stock photos,uploadvr,upload photos to icloud,mass spectrometry instruments ltd,free music,upload video to youtube,upload speed test,upload photo,profiling beds,video phone ft lady gaga,profiling definition,mass spectrometry basics,profiling in spring boot,freep,video phone beyonce,sharingan naruto,upload download speed,mass spectrometry machine,video phone extended remix,video phone call,sharingbox,upload youtube,upload to google drive,freeform,mass spectrometry graph,profiling meaning,sharing economy,sharing is caring meme,upload resume to linkedin,mass spectrometry imaging,sharing wifi password,sharing kindle books,sharing community yonkers,app video iphone,free vpn,sharing wifi iphone,mass spectrometry fragmentation,free fonts,upload music to spotify,video phone screen,upload pictures,mass spectrometry a level questions,free people,profiling python code,free online games,free movies online,video phone wikipedia,sharing outlook calendar,sharing network 5k,profiling paris,freecell,video phone gaga,video phone beyonce lyrics,mass spectrometry a level,upload soundcloud,freebirds,profiling tools,video phone beyonce letra,profiling synonym,upload pdf to kindle,free movies,profiling techniques,upload studio,freestyle libre,sharing network,sharingan contacts,upload image,video phone lyrics,upload to instagram from pc,mass spectrometry questions,profiling test,upload file,mass spectrometry analysis,profiling people,mass spectrometry gcse,free games,video phone tradução,mass spectrometry jobs uk,profiling gdpr,mass spectrometry pdf,sharing google calendar,video phone lady gaga,profiling data,mass spectrometry oxford,upload video,video phone feat lady gaga,mass spectrometry chemguide,sharingan,sharingan tattoo,mass spectrometry proteomics,video phone beyonce lady gaga,mass spectrometry steps,mass spectrometry a level chemistry,profiling form,profiling books,free credit report,free credit score,
Không có nhận xét nào:
Đăng nhận xét